Polymyositis Research Association

List of antibodies for detecting ER stress markers

List of primers for detecting ER stress markers by real-time PCR

Gen	Human	Mouse	Rat
sXBP1	CTGAGTCCGAATCAGGTGCAG ATCCATGGGGAGATGTTCTGG	CTGAGTCCGAATCAGGTGCAG GTCCATGGGAAGATGTTCTGG	CTGAGTCCGAATCAGGTGCAG ATCCATGGGAAGATGTTCTGG
usXBP1	CAGCACTCAGACTACGTGCA ATCCATGGGGAGATGTTCTGG	CAGCACTCAGACTATGTGCA GTCCATGGGAAGATGTTCTGG	CAGCACTCAGACTACGTGCG ATCCATGGGAAGATGTTCTGG
Total XBP1	TGGCCGGGTCTGCTGAGTCCG ATCCATGGGGAGATGTTCTGG	TGGCCGGGTCTGCTGAGTCCG GTCCATGGGAAGATGTTCTGG	TGGCCGGGTCTGCTGAGTCCG ATCCATGGGAAGATGTTCTGG
ATF4	GTTCTCCAGCGACAAGGCTA ATCCTGCTTGCTGTTGTTGG	GGGTTCTGTCTTCCACTCCA AAGCAGCAGAGTCAGGCTTTC	AATGGATGACCTGGAAACCA TCTTGGACTAGAGGGGCAAA
CHOP	AGAACCAGGAAACGGAAACAGA TCTCCTTCATGCGCTGCTTT	CCACCACACCTGAAAGCAGAA AGGTGAAAGGCAGGGACTCA	AGAGTGGTCAGTGCGCAGC CTCATTCTCCTGCTCCTTCTCC
BiP	TGTTCAACCAATTATCAGCAAACTC TTCTGCTGTATCCTCTTCACCAGT	TTCAGCCAATTATCAGCAAACTCT TTTTCTGATGTATCCTCTTCACCAGT	TGGGTACATTTGATCTGACTGGA CTCAAAGGTGACTTCAATCTGGG
GRP94	GAAACGGATGCCTGGTGG GCCCCTTCTTCCTGGGTC	AAGAATGAAGGAAAAACAGGACAAAA CAAATGGAGAAGATTCCGCC
EDEM	CAAGTGTGGGTACGCCACG AAAGAAGCTCTCCATCCGGTC	CTACCTGCGAAGAGGCCG GTTCATGAGCTGCCCACTGA

Footnotes

Christine M. Measuring ER stress and the unfolded protein response using mammalian tissue culture system .Author manuscript; available in PMC 2013 Jul 4. Published in final edited form as: Methods Enzymol. 2011; 490: 71–92. doi: 10.1016/B978-0-12-385114-7.00004-0 PMCID: PMC3701721 NIHMSID: NIHMS45736
Reimold AM, Etkin A, Clauss I, Perkins A, Friend DS, Zhang J, Horton HF, Scott A, Orkin SH, Byrne MC, Grusby MJ, Glimcher LH. Genes Dev. 2000;14:152–157. [PMC free article] [PubMed]
Reimold AM, Iwakoshi NN, Manis J, Vallabhajosyula P, Szomolanyi-Tsuda E, Gravallese EM, Friend D, Grusby MJ, Alt F, Glimcher LH. Nature. 2001;412:300–307. [PubMed]
Harding HP, Zhang Y, Zeng H, Novoa I, Lu PD, Calfon M, Sadri N, Yun C, Popko B, Paules R, Stojdl DF, Bell JC, Hettmann T, Leiden JM, Ron D. Mol Cell. 2003;11:619–633. [PubMed]
Novoa I, Zhang Y, Zeng H, Jungreis R, Harding HP, Ron D. Embo J. 2003;22:1180–1187. [PMC free article] [PubMed]
Miyazaki J, Araki K, Yamato E, Ikegami H, Asano T, Shibasaki Y, Oka Y, Yamamura K. Endocrinology. 1990;127:126–132. [PubMed]
Asfari M, Janjic D, Meda P, Li G, Halban PA, Wollheim CB. Endocrinology. 1992;130:167–178. [PubMed]
Hohmeier HE, Mulder H, Chen G, Henkel-Rieger R, Prentki M, Newgard CB.